Monday . . . .
Tuesday . . . . 9:30 - 10:30
Wednesday . . 9:30 - 10:30
Thursday. . . .
Friday. . . . . . Others by appt.
Biographical Information:David Pennock is a molecular cell biologist who is interested in mechanisms of ciliary motility. Cilia and flagella use multiple, different types of dyneins to drive movement of the organelle. Dr. Pennock is interested in how the different dyneins are organized along the axoneme and in the role(s) each different dynein plays in ciliary motility. To address those problems, Dr. Pennock and his students clone and analyze dynein heavy chain genes from the unicellular protozoan, Tetrahymena thermophila. They introduce mutations into different dynein heavy chain genes in Tetrahymena by targeted gene knockout. They analyze the phenotypes of the mutants in order to infer the functions of each dynein. Students in the Pennock lab have introduced mutations into both outer and inner arm dynein heavy chain genes.
Dr. Pennock and his students use molecular biological techniques to clone and manipulate the dynein heavy chain genes in vitro and biolistics to put the manipulated genes back into Tetrahymena. His students use biochemical techniques to analyze ciliary proteins, high speed video light microscopy to investigate cell behavior and electron microscopy to study the organization of dyneins in ciliary axonemes. Thus, research in Dr. Pennock's laboratory extends from the gene to the organism, which offers his students the opportunity to learn and use techniques that range from molecular biology to biochemistry to microscopy.